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cm2 native page gels  (Hoefer)
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Hoefer cm2 native page gels
Fig. 1 A schematic representation of the in-gel immunodetection method for the detection of cocaine on banknotes. The numbers indicate as follows; 1. the banknote is submerged in a polyacrylamide gel solution; 2. a 16 × 18 <t>cm2</t> cassette containing the polyacrylamide gel solution; 3. the gel is set at room temperature; 4. the gel, with the encased banknote, is removed from the cassette; 5. the gel is placed in 50% isopropanol in dH2O; 6. incubation followed by washing; 7. the HRP-labelled anti-cocaine antibody is added; 8. the antibody passes through the gel and binds to the cocaine on the banknote; 9. washing; 10. the ECL substrate is added; 11. an incubation step (in the dark) and washing; 12. chemiluminescent imaging. Figure adapted from van der Heide and Russell.41
Cm2 Native Page Gels, supplied by Hoefer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 A schematic representation of the in-gel immunodetection method for the detection of cocaine on banknotes. The numbers indicate as follows; 1. the banknote is submerged in a polyacrylamide gel solution; 2. a 16 × 18 cm2 cassette containing the polyacrylamide gel solution; 3. the gel is set at room temperature; 4. the gel, with the encased banknote, is removed from the cassette; 5. the gel is placed in 50% isopropanol in dH2O; 6. incubation followed by washing; 7. the HRP-labelled anti-cocaine antibody is added; 8. the antibody passes through the gel and binds to the cocaine on the banknote; 9. washing; 10. the ECL substrate is added; 11. an incubation step (in the dark) and washing; 12. chemiluminescent imaging. Figure adapted from van der Heide and Russell.41

Journal: The Analyst

Article Title: Location-specific immunodetection of cocaine on banknotes.

doi: 10.1039/c6an01398h

Figure Lengend Snippet: Fig. 1 A schematic representation of the in-gel immunodetection method for the detection of cocaine on banknotes. The numbers indicate as follows; 1. the banknote is submerged in a polyacrylamide gel solution; 2. a 16 × 18 cm2 cassette containing the polyacrylamide gel solution; 3. the gel is set at room temperature; 4. the gel, with the encased banknote, is removed from the cassette; 5. the gel is placed in 50% isopropanol in dH2O; 6. incubation followed by washing; 7. the HRP-labelled anti-cocaine antibody is added; 8. the antibody passes through the gel and binds to the cocaine on the banknote; 9. washing; 10. the ECL substrate is added; 11. an incubation step (in the dark) and washing; 12. chemiluminescent imaging. Figure adapted from van der Heide and Russell.41

Article Snippet: 16 × 18 cm2 native-PAGE gels were cast using a Hoefer SE600 dual cassette.

Techniques: Immunodetection, Incubation, Imaging